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. 2017 May 24;38(1):21–30. doi: 10.3892/or.2017.5668

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Copyright: © Bhat et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — GROα is overexpressed in TNBC cells. Baseline GROα expression levels in breast cancer subtypes and 17 additional TNBC cell lines were evaluated by (A) q-PCR by extracting total RNA and presented as GROα/18S ratio whose average fold was normalized to MCF7 GROα levels (B) ELISA by collecting cell supernatants containing secreted GROα from breast cancer cells (C) ELISA by collecting cell lysates from breast cancer cells. The GROα concentration levels were normalized per microgram (µg) of the total protein. Experiments were performed thrice with triplicates per experimental analysis. Bars correspond to mean ± SD.