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. 2017 Jun 29;17:342. doi: 10.1186/s12906-017-1852-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — Immunocytochemistry demonstrates the expression of surface antigen (TRA-1-60) in NT2 cells. a NT2 cells treated with RS1S CHCL3 extract show downregulation of TRA-1-60 expression. b NT2 cells treated with 0.001% MeOH as a vehicle control (VC) show high expression of TRA-1-60. c Goat anti-mouse (IgM-FITC) as a negative control. Nuclei stained by Hoechst (blue)