Fig. 5. Cell surface binding of AF633-labeled HA capsules with different HA layers to HeLa cells, as determined by deconvolution microscopy. (a) Images of cells bound with capsules. (b) Percentage of capsule volume colocalized with cell membrane, analyzed via Imaris software. Cells were incubated with capsules (red) at a capsule to cell ratio of 100 : 1 for 2 h at 4 °C. Cell membranes were stained with AF488-WGA (green). Scale bars are 10 μm.
