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. Author manuscript; available in PMC: 2018 May 22.
Published in final edited form as: Dev Cell. 2017 May 22;41(4):366–381.e4. doi: 10.1016/j.devcel.2017.04.014

Figure 4. S1P regulates integrinβ8 mRNA in LGE neural progenitors in a region-specific manner dependent on Ric8a/p38MAPK.

Figure 4

See also Figure S4.

(A) S1P dramatically up-regulates β8 mRNA in LGE neurospheres, an effect blocked by p38 inhibitor and ric8a mutation. *, p < 0.05; n = 5-7.

(B) S1PR1 antagonist W146 suppresses basal and S1P-induced β8 mRNA in LGE neurospheres. **, p < 0.01; n = 4.

(C) W146 has no effects on αv mRNA in LGE neurospheres (p > 0.05; n = 4).

(D–E) S1P does not induce β8 mRNA in MGE (D) or cortical (E) neurospheres (p > 0.45; n = 4).

(F) s1pr1/ric8a compound mutation suppresses basal and S1P-induced β8 mRNA in LGE neurospheres. *, p < 0.05; **, p < 0.01; n = 3.

(G) S1P treatment (10 minutes) specifically induces p38 phosphorylation in LGE, but not cortical or MGE neurospheres.