FIG. 1.

Identification of antigenic regions of Tc40 by immunoblot analysis. A schematic representation of the Tc40 protein (916 amino acids) is shown at the top. Regions encoding the amino-terminal domain (N), the central domain (CD), or the carboxy-terminal domain (C) of Tc40 were expressed in E. coli as GST fusion proteins. Fusion proteins were separated by SDS-PAGE, transferred onto nitrocellulose membranes, and incubated with rabbit antiserum against GST (A) or a pool of human chronic chagasic sera (B). Lanes: 1, N-terminal domain of Tc40; 2, C-terminal domain of Tc40; 3, central domain of Tc40; 4, GST without fusion.