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. Author manuscript; available in PMC: 2017 Jun 30.
Published in final edited form as: Bioconjug Chem. 2016 Oct 14;28(1):203–211. doi: 10.1021/acs.bioconjchem.6b00529

Scheme 1. Workflow for Simultaneous Capture and Detection of Nucleic Acid and Protein Target Molecules Using biotin—PEG AuNP Probesa.

Scheme 1

aBiomolecules are biotinylated and added to microarray slides containing capture molecules (complementary nucleic acid sequences or primary capture antibodies). Streptavidin and biotin—PEG—AuNPs are subsequently applied to the slide and then amplified with 1 mM HAuCl4 and 5 mM NH2OH to detect the captured targets.