Table 1.
Overview of Kinetic Rates and Quantities Estimated in This Study
| Symbol | Definition | Estimate |
|---|---|---|
| R | cellular FLC mRNA counts (in outer layer cells) | average: 58 ± 2; min–max: 7–145 |
| V | cell volume (in outer layer cells) | average: 1.8 pL; min–max: 0.5–4 pL |
| β | slope of linear relationship between cellular FLC mRNA count and cell volume | 31 ± 1 pL−1 |
| d | mRNA degradation rate | 3.3 ± 0.1 × 10−5 s−1 |
| p | cellular mRNA production rate | average: 1.8 × 10−3 s−1; min–max: 0.5–4 × 10−3 s−1 |
| Nloci | cell cycle averaged number of FLC loci per cell | 2.5; range: 2.4–2.8 |
| F | cell cycle averaged FLC transcription initiation rate | 7.5 ± 0.4 × 10−4 s−1 |
| v | Pol II elongation rate | 2–20 bp/s |
| σ | intron processing rate | 1.0–2.0 × 10−3 s−1 |
| k53 | intron lariat 5′ to 3′ degradation rate | 1–5 bp/s |
| k35 | intron lariat 3′ to 5′ degradation rate | 1–4 bp/s |
| Rloc | FLC mRNA levels at locus | average: 2.2 ± 0.3; min–max: 0–6 |
| kx | mRNA release rate from locus | 3.4–5 × 10−4 s−1 |
| bs | burst size in ON/OFF model | 1–3 |
| koff | transition rate from ON to OFF state in ON/OFF model | 0.1 s−1 |