REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-DIG-fluorescein antibody	Roche	Cat#11207741910; RRID: AB_514498
Chemicals, Peptides, and Recombinant Proteins
Digoxigenin-dUTP	Roche	Cat#11745816910
Actinomycin D	SigmaTM	Cat# A4262-2MG
Glucose oxidase	Sigma TM	Cat# G0543
Bovine liver catalase	Sigma TM	Cat# C3155
Experimental Models: Organisms/Strains
Arabidopsis ColFRI: Col-0 ecotype with an introgressed active FRIGIDA allele from the Spanish San Feliu 2 (Sf2) accession.	Caroline Dean laboratory, first described in (Lee et al., 1994).	N/A
Arabidopsis FLC-TEX: transformation of 12-kb FLC genomic DNA fragment composed of the promoter region, gene body, and replacement of the 3′ region by rbcs3B terminator into the flc-2 FRI genotype. flc-2 is a loss-of-function FLC genotype, which has a deletion/rearrangement within the endogenous FLC gene. A representative FLC-TEX line (no. 577) was selected.	Caroline Dean laboratory, first described in (Wang et al., 2014)	N/A
Arabidopsis FLC-CTL: transformation of FLC genomic construct (15 kb of the FLC locus, FLC-15) into flc-2 FRI background.	Caroline Dean laboratory, first described in (Csorba et al., 2014)	N/A
Oligonucleotides
smFISH probes, see Table S1	Biosearch Technologies	N/A
Software and Algorithms
Cellular RNA count and Z-projected cell area script	Duncan et al., 2016, Olsson and Hartley, 2016	https://github.com/ri23/FISHmodel
Cell volume estimation script	This study	https://github.com/ri23/FISHmodel
Cellular mRNA production and degradation simulations	This study	https://github.com/ri23/FISHmodel
FLC transcription and RNA processing simulations	This study	https://github.com/ri23/FISHmodel
FISH-quant	Mueller et al., 2013	https://code.google.com/archive/p/fish-quant/