REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
anti-DIG-fluorescein antibody | Roche | Cat#11207741910; RRID: AB_514498 |
Chemicals, Peptides, and Recombinant Proteins | ||
Digoxigenin-dUTP | Roche | Cat#11745816910 |
Actinomycin D | SigmaTM | Cat# A4262-2MG |
Glucose oxidase | Sigma TM | Cat# G0543 |
Bovine liver catalase | Sigma TM | Cat# C3155 |
Experimental Models: Organisms/Strains | ||
Arabidopsis ColFRI: Col-0 ecotype with an introgressed active FRIGIDA allele from the Spanish San Feliu 2 (Sf2) accession. | Caroline Dean laboratory, first described in (Lee et al., 1994). | N/A |
Arabidopsis FLC-TEX: transformation of 12-kb FLC genomic DNA fragment composed of the promoter region, gene body, and replacement of the 3′ region by rbcs3B terminator into the flc-2 FRI genotype. flc-2 is a loss-of-function FLC genotype, which has a deletion/rearrangement within the endogenous FLC gene. A representative FLC-TEX line (no. 577) was selected. | Caroline Dean laboratory, first described in (Wang et al., 2014) | N/A |
Arabidopsis FLC-CTL: transformation of FLC genomic construct (15 kb of the FLC locus, FLC-15) into flc-2 FRI background. |
Caroline Dean laboratory, first described in (Csorba et al., 2014) | N/A |
Oligonucleotides | ||
smFISH probes, see Table S1 | Biosearch Technologies | N/A |
Software and Algorithms | ||
Cellular RNA count and Z-projected cell area script | Duncan et al., 2016, Olsson and Hartley, 2016 | https://github.com/ri23/FISHmodel |
Cell volume estimation script | This study | https://github.com/ri23/FISHmodel |
Cellular mRNA production and degradation simulations | This study | https://github.com/ri23/FISHmodel |
FLC transcription and RNA processing simulations | This study | https://github.com/ri23/FISHmodel |
FISH-quant | Mueller et al., 2013 | https://code.google.com/archive/p/fish-quant/ |