| UltraPure formamide | 25mL |
| 1M Tris/HCl, pH 7.6 | 500μL |
| 10mg/ml tRNA | 1mL |
| 50X Denhardt’s solution | 1mL |
| 50% Dextran Sulfate | 10mL |
| 5M NaCl | 6mL |
| 10% SDS | 1.25mL |
| DEPC-treated water | 5.25mL |
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