FIG. 1.

Hypersensitivity to MMS cosegregates with the tagging rs-3 marker. (A) Growth and survival of cells on TAP medium or on TAP medium containing 2.5 mM MMS (TAP + MMS). The parental strains and the meiotic products of three tetrads from the cross between the wild-type (CC-125) and mutant Ble-1 strains are shown. Strains grown to logarithmic phase were diluted in TAP medium to 5 × 10⁴ cells per 5 μl, spotted on plates, and incubated as described in Materials and Methods. CTRL, control. (B) Southern blot analysis of the indicated strains. Genomic DNA was digested with HindIII and probed with the pBluescript sequence, the vector backbone of the rs-3 tagging plasmid.