FIG. 3.

Influence of rop1 and hmg3 deletions on conjugation tube formation. (A) The FB1 (a1 b1) wild-type (wt) and mutant strains indicated on top were stimulated with a2 pheromone for 5 h (lower panel) or treated with dimethyl sulfoxide for the same period of time (upper panel). All pictures were taken at the same magnification. Bar, 20 μm. (B) All strains indicated on top are derivatives of FB1Pcrg1::fuz7DD, encoding an arabinose-inducible, constitutively active allele of the MAPKK Fuz7. Cell morphology after 5 h in arabinose-containing medium (lower panels) was compared to that of cells grown with glucose as the carbon source for the same period of time (upper panels). All pictures were taken at the same magnification. Bar, 20 μm.