FIG. 4.

rop1 is required for pheromone-responsive gene expression. RNA was prepared from the strains listed on top and subjected to Northern analysis. (A) All strains indicated on top are FB1 (a1 b1) derivatives. Cells were either untreated (lanes 1 and 2) or treated for 5 h with synthetic a2 pheromone dissolved in dimethyl sulfoxide (+) or with the same volume of dimethyl sulfoxide (−) (lanes 3 to 6); 10 μg of total RNA was loaded per lane. The blot was probed successively with the gene probes indicated on the right. (B) All strains used are derivatives of strain FB1Pcrg1::fuz7DD. Strains were grown with glucose (−) or arabinose (+) as the carbon source. RNA was isolated, and 12 μg of total RNA was loaded per lane. The filter was hybridized in succession with the gene probes indicated on the right. The rRNA probe served as a loading control. Arrowheads mark the three rop1 transcripts.