TABLE 2.
Influence of rop1 deletion on UAS activity and pheromone induction of the prf1 promoter
| Strain | Relative fluorescencea (±SEM)
|
|||
|---|---|---|---|---|
| NM-glucose | NM-maltose | Control | Pheromone | |
| HA232 | 1.00 (±0.09) | 0.13 (±0.02) | ND | ND |
| HA232Δrop1 | 0.98 (±0.07) | 0.15 (±0.02) | ND | ND |
| FB1Pprf1:gfp | ND | ND | 1.00 (±0.03) | 36.02 (±0.34) |
| FB1Δrop1Pprf1:gfp | ND | ND | 0.19 (±0.03) | 0.11 (±0.02) |
| FB2Pprf1:gfp | ND | ND | 1.00 (±0.10) | 13.28 (±0.09) |
| FB2Δrop1Pprf1:gfp | ND | ND | 0.45 (±0.19) | 0.40 (±0.06) |
a
Relative fluorescence was calculated from measured GFP fluorescence per OD600. The average relative fluorescence of the wild-type strains (HA232, FB1Pprf1:gfp, and FB2Pprf1:gfp) grown in glucose-containing medium or treated with dimethyl sulfoxide (control) was set to 1.