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. 2005 Feb;4(2):281–288. doi: 10.1128/EC.4.2.281-288.2005

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FIG. 1. — Btn2p interacts with Ist2p in vivo. Immunoprecipitation was performed with anti-FLAG antibody and cell extracts derived from strain YPH499 expressing Btn2p-FLAG or both Btn2p-FLAG and Ist2p-c-myc. (Upper panel) Western analysis performed with anti-c-myc monoclonal antibody. (Lower panel) Western analysis performed with anti-FLAG monoclonal antibody. Lanes: 1, cell extract of YPH499 expressing Btn2p-FLAG; 2, cell extract of YPH499 expressing Btn2p-FLAG and immunoprecipitated with anti-FLAG antibody; 3, cell extract of YPH499 expressing Btn2p-FLAG and Ist2p-c-myc; 4, cell extract of YPH499 expressing Btn2p-FLAG and Ist2p-c-myc and immunoprecipitated with anti-FLAG antibody. Size markers are indicated on the right. The arrow indicates the Ist2p-c-myc band.