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. 2017 Jun 30;12(6):e0179012. doi: 10.1371/journal.pone.0179012

Fig 2. Macrophage Migration Inhibitory Factor (MIF) is a critical component of GCM promoting MDSC formation.

Fig 2

A) ELISA results confirming the presence of MIF in glioma-conditioned media. An MIF neutralizing antibody blocks the formation of CD14+/HLA-DR- cells when cultured in hypoxic (1% O2) GCM. B) Representative dot plots showing CD 14 and HLA-DR expression in monocytes in serum free DMEM (top left), GCM from BT 116 (top right), GCM + 20 ug/ml anti-MIF Ab (bottom left), and GCM + 40 ug/ml anti-MIF Ab (bottom right). There is a reduction in the amount of CD14+/HLA-DR− cells (mMDSC’s) in the anti-MIF treated monocytes (percent shown in red). C) Bar graphs showing a significant reduction in the mean frequency of CD14+/HLA-DR- cells generated from monocytes cultured in GCM in the presence of anti-MIF antibodies. **** = P < 0.0001.