Table 1. Expression of biomarkers in glioma conditioned media.
Name | DMEM | 114 GCM | 116 GCM | 120 GCM |
---|---|---|---|---|
Angiogenin | 0 | 2676 | 3454 | 2668 |
BDNF | 0 | 0 | 0 | 131 |
Eotaxin-3 | 0 | 0 | 0 | 0 |
Flt-3 Ligand | 0 | 0 | 3 | 50 |
G-CSF | 0 | 0 | 0 | 0 |
GDNF | 0 | 0 | 0 | 0 |
GM-CSF | 0 | 0 | 0 | 155 |
GRO | 0 | 0 | 111 | 749 |
GRO-alpha | 0 | 0 | 0 | 0 |
HGF | 0 | 0 | 1026 | 0 |
IFN-gamma | 0 | 0 | 0 | 0 |
IGF-I | 0 | 0 | 0 | 7 |
IGFBP-1 | 0 | 0 | 0 | 109 |
IGFBP-2 | 0 | 398 | 1062 | 636 |
IGFBP-3 | 0 | 0 | 559 | 152 |
IL-1 alpha | 0 | 0 | 0 | 0 |
IL-1 beta | 0 | 0 | 0 | 122 |
IL-10 | 0 | 0 | 0 | 0 |
IL-12 p70 | 0 | 0 | 0 | 0 |
IL-13 | 0 | 0 | 0 | 0 |
IL-15 | 0 | 0 | 0 | 0 |
IL-2 | 0 | 0 | 0 | 0 |
IL-3 | 0 | 0 | 0 | 0 |
IL-5 | 0 | 0 | 0 | 0 |
IL-6 | 0 | 178 | 2157 | 9665 |
IL-7 | 0 | 0 | 0 | 0 |
IL-8 | 0 | 103 | 5303 | 15294 |
IP-10 | 0 | 5 | 0 | 0 |
LIF | 0 | 0 | 0 | 0 |
MCP-1 | 0 | 2993 | 40301 | 31031 |
MCP-2 | 0 | 0 | 0 | 0 |
MCSF | 0 | 0 | 0 | 0 |
MIF | 0 | 1932 | 4254 | 9857 |
MIG | 0 | 0 | 0 | 0 |
MIP-3 alpha | 0 | 0 | 0 | 88 |
Oncostatin M | 0 | 0 | 0 | 0 |
Osteopontin | 0 | 0 | 1851 | 225 |
Osteoprotegerin | 0 | 0 | 0 | 560 |
PARC | 0 | 0 | 0 | 0 |
PDGF-BB | 0 | 0 | 0 | 86 |
PIGF | 0 | 0 | 140 | 0 |
RANTES | 0 | 0 | 0 | 319 |
SCF | 0 | 99 | 0 | 91 |
SDF-1 | 0 | 0 | 0 | 0 |
TGF-beta 1 | 0 | 0 | 0 | 7 |
TGF-beta 2 | 0 | 0 | 0 | 0 |
TIMP-1 | 0 | 601 | 2539 | 1982 |
TIMP-2 | 0 | 8430 | 6869 | 8951 |
TNF-alpha | 0 | 0 | 0 | 0 |
TNF-beta | 0 | 0 | 0 | 0 |
VEGF | 0 | 0 | 781 | 636 |
Cytokine expression in GCM. A cytokine array demonstrates a panel of 80 cytokines from fresh media and glioma-conditioned media derived from three human glioma cell lines (BT114, BT116, BT120). Numbers represent mean fluorescent units. All numbers were zeroed to the fresh media which served as a control. Red fields represent values greater than 2 standard deviations above the negative standard mean. Green fields represent non-significant positive values. Note: these cell lines are all IDH-wild type but have not been extensively characterized genetically otherwise.