Table 1. Expression of biomarkers in glioma conditioned media.
| Name | DMEM | 114 GCM | 116 GCM | 120 GCM |
|---|---|---|---|---|
| Angiogenin | 0 | 2676 | 3454 | 2668 |
| BDNF | 0 | 0 | 0 | 131 |
| Eotaxin-3 | 0 | 0 | 0 | 0 |
| Flt-3 Ligand | 0 | 0 | 3 | 50 |
| G-CSF | 0 | 0 | 0 | 0 |
| GDNF | 0 | 0 | 0 | 0 |
| GM-CSF | 0 | 0 | 0 | 155 |
| GRO | 0 | 0 | 111 | 749 |
| GRO-alpha | 0 | 0 | 0 | 0 |
| HGF | 0 | 0 | 1026 | 0 |
| IFN-gamma | 0 | 0 | 0 | 0 |
| IGF-I | 0 | 0 | 0 | 7 |
| IGFBP-1 | 0 | 0 | 0 | 109 |
| IGFBP-2 | 0 | 398 | 1062 | 636 |
| IGFBP-3 | 0 | 0 | 559 | 152 |
| IL-1 alpha | 0 | 0 | 0 | 0 |
| IL-1 beta | 0 | 0 | 0 | 122 |
| IL-10 | 0 | 0 | 0 | 0 |
| IL-12 p70 | 0 | 0 | 0 | 0 |
| IL-13 | 0 | 0 | 0 | 0 |
| IL-15 | 0 | 0 | 0 | 0 |
| IL-2 | 0 | 0 | 0 | 0 |
| IL-3 | 0 | 0 | 0 | 0 |
| IL-5 | 0 | 0 | 0 | 0 |
| IL-6 | 0 | 178 | 2157 | 9665 |
| IL-7 | 0 | 0 | 0 | 0 |
| IL-8 | 0 | 103 | 5303 | 15294 |
| IP-10 | 0 | 5 | 0 | 0 |
| LIF | 0 | 0 | 0 | 0 |
| MCP-1 | 0 | 2993 | 40301 | 31031 |
| MCP-2 | 0 | 0 | 0 | 0 |
| MCSF | 0 | 0 | 0 | 0 |
| MIF | 0 | 1932 | 4254 | 9857 |
| MIG | 0 | 0 | 0 | 0 |
| MIP-3 alpha | 0 | 0 | 0 | 88 |
| Oncostatin M | 0 | 0 | 0 | 0 |
| Osteopontin | 0 | 0 | 1851 | 225 |
| Osteoprotegerin | 0 | 0 | 0 | 560 |
| PARC | 0 | 0 | 0 | 0 |
| PDGF-BB | 0 | 0 | 0 | 86 |
| PIGF | 0 | 0 | 140 | 0 |
| RANTES | 0 | 0 | 0 | 319 |
| SCF | 0 | 99 | 0 | 91 |
| SDF-1 | 0 | 0 | 0 | 0 |
| TGF-beta 1 | 0 | 0 | 0 | 7 |
| TGF-beta 2 | 0 | 0 | 0 | 0 |
| TIMP-1 | 0 | 601 | 2539 | 1982 |
| TIMP-2 | 0 | 8430 | 6869 | 8951 |
| TNF-alpha | 0 | 0 | 0 | 0 |
| TNF-beta | 0 | 0 | 0 | 0 |
| VEGF | 0 | 0 | 781 | 636 |
Cytokine expression in GCM. A cytokine array demonstrates a panel of 80 cytokines from fresh media and glioma-conditioned media derived from three human glioma cell lines (BT114, BT116, BT120). Numbers represent mean fluorescent units. All numbers were zeroed to the fresh media which served as a control. Red fields represent values greater than 2 standard deviations above the negative standard mean. Green fields represent non-significant positive values. Note: these cell lines are all IDH-wild type but have not been extensively characterized genetically otherwise.