FIG. 3.

Growth and hydrogen peroxide sensitivity of thioredoxin mutants. (A) Wild-type and trx1, trx2, trx3, trx1 trx2, trx1 trx3, trx2 trx3, and trx1 trx2 trx3 mutant cells were inoculated to the same initial cell density in minimal glucose medium (SD; initial A₆₀₀ = 0.0025) or minimal medium containing glycerol plus ethanol (SGE; initial A₆₀₀ = 0.05), and growth was monitored by A₆₀₀. (B) Sensitivity to hydrogen peroxide was determined by spotting the same strains onto YEPD plates containing 4 mM H₂O₂. Cultures of strains were grown into exponential phase or stationary phase and adjusted to an A₆₀₀ of 1.0 or 0.1 before spotting onto appropriate plates. Plates were incubated at 30°C for 3 days before scoring growth.