FIG. 7.
Growth and hydrogen peroxide sensitivity of GSH reductase mutants. The indicated strains were inoculated to the same initial cell density in minimal glucose medium (SD; initial A600 = 0.0025) or minimal medium containing glycerol plus ethanol (SGE; initial A600 = 0.05), and growth was monitored by A600. (B) Sensitivity to hydrogen peroxide was determined by spotting the same strains onto YEPD plates containing 4 mM H2O2. Cultures of strains were grown into stationary phase and adjusted to an A600 of 1.0 or 0.1 before spotting onto appropriate plates. Plates were incubated at 30°C for 3 days before scoring growth.