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. Author manuscript; available in PMC: 2017 Jun 30.
Published in final edited form as: Nat Neurosci. 2016 Oct 17;19(12):1636–1646. doi: 10.1038/nn.4414

Figure 1. Activity-dependent transcriptional profiling of BLA neurons.

Figure 1

a, Viral-based genetic scheme for activity-dependent transcriptional profiling. c-Fos promoter activity drives the expression of tTA, which in turn, binds TRE and drives the expression of PABP-FLAG in the absence of doxycycline (Dox). b, PABP-FLAG expression in the BLA in mice kept on a Dox diet (On Dox), taken off a Dox diet and exposed to home cage (Off Dox), Shock, Female, Seizure, (one-way ANOVA, F4,25=131.0, P<0.0001, n = 6 per group). Significance for multiple comparisons, **P < 0.01, ****P < 0.0001, not significant (N.S.). c, PABP-FLAG expression in soma and varicosities of a BLA neuron. FLAG expression in the BLA of On Dox (d), Off Dox (e), Seizure (f), Shock (g,h), and Female (i,j) group. FLAG expression and nuclear marker, DAPI, in Shock (h), and Female (j) group. Scale bar 25μm (c), 250μm (d,e,f,g,i), 80 μm (h,j). k, RMA normalizedn RNA expression values from microarray from RNA purified from Shock (n = 3) and Female (n = 3) groups. Red and green points represent enriched genes (>1.25 fold, ANOVA p<.05, log2 scale). l, Quantification of in situ hybridization of BLA expression of candidate genetic markers enriched in shock group (green) and female group (red) (n = 3 mice per group). Positive control genes (black). Results show mean ± s.e.m (b,l).