Fig 5. Activities of MtuCM, wild type and variant MtuDAH7PS.

(A) Activation of MtuCM activity in the presence of ten-fold molar excess of wild-type MtuDAH7PS, MtuDAH7PS_G232P or MtuDAH7PS_F227D. The dashed line indicates the activity of MtuCM alone. Assays conducted with 150 μM chorismic acid and 60 nM MtuCM except for MtuCM alone where 90 nM was used. Specific activity of (B) wild-type MtuDAH7PS and (C) MtuDAH7PS_G232P and (D) MtuDAH7PS_F227D in the presence of various single and binary combinations of aromatic amino acids. For binary combinations, the background concentration of the indicated ligand was held at 100 μM. Assays conducted in the presence of 150 μM PEP and 150 μM E4P.