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. 2017 Jun 30;12(6):e0180052. doi: 10.1371/journal.pone.0180052

Table 1. Kinetic parameters of wild-type MtuDAH7PS, MtuDAH7PSG232P and MtuDAH7PSF227D determined from ternary complex rate equation.

MtuDAH7PS KmE4P
(μM)
KmPEP
(μM)
kcat
(s-1)
Wild-type 29 ± 5 15 ± 4 6.7 ± 0.4
G232P 330 ± 10 90 ± 20 15 ± 7
F227D 590 ± 60 80 ± 20 8 ± 3