Table 1. Kinetic parameters of wild-type MtuDAH7PS, MtuDAH7PSG232P and MtuDAH7PSF227D determined from ternary complex rate equation.
MtuDAH7PS |
KmE4P (μM) |
KmPEP (μM) |
kcat (s-1) |
---|---|---|---|
Wild-type | 29 ± 5 | 15 ± 4 | 6.7 ± 0.4 |
G232P | 330 ± 10 | 90 ± 20 | 15 ± 7 |
F227D | 590 ± 60 | 80 ± 20 | 8 ± 3 |