Table 3. Interface analysis results for MtuDAH7PS WT and G232P from PISA [29].
ΔiG indicates the solvation free energy gain upon formation of the interface. P-value indicates the probability to obtain solvation energy gain lower than the observed value ΔiG, if interface atoms were picked randomly from protein surface such as to amount to the observed interface area. An interface with P>0.5 is likely to be an artefact and interfaces with P<0.5 are likely to be interaction specific. NNH and NSB indicate the number of potential hydrogen bonds and salt bridge interactions across the interface, respectively.
| Dimer interface | (Pseudo) tetramer interface | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Interface area (Å2) | ΔiG (kcal/mol) | ΔiG P-value | NHB | NSB | Interface area (Å) | ΔiG (kcal/mol) | ΔiG P-value | NHB | NSB | |
| MtuDAH7PSWT | 2054.4 | -25.7 | 0.220 | 28 | 15 | 915.1 | -15.9 | 0.087 | 4 | 1 |
| MtuDAH7PSG232P | 2123.6 | -27.8 | 0.240 | 28 | 10 | 839.7 | -6.8 | 0.651 | 27 | 6 |