Table 4. SAXS parameters calculated from analysis of the scattering profiles of MtuDAH7PS variants using PRIMUS, GNOM, MoW, and CRYSOL.
Values were obtained by analysis of one set of SAXS data.
| MtuDAH7PSWT | MtuDAH7PSG232P | MtuDAH7PSF227D | |
|---|---|---|---|
| Data points | 379 | 207 | 297 |
| q-range | 0.014–0.265 | 0.019–0.269 | 0.014–0.269 |
| Guinier analysis | |||
| Rg (Å) | 41.6 ± 0.3 | 29.8 ± 0.1 | 29.8 ± 0.1 |
| I (0) (cm-1) | 0.168 ± 0.001 | 0.074 ± 0.001 | 0.066 ± 0.001 |
| Pair distribution analysis | |||
| Real Space Rg (Å) | 40.9 ± 0.2 | 29.6 ± 0.1 | 28.83 ± 0.07 |
| Dmax (Å) | 127 | 98 | 96 |
| Vp, (Da) | 282700 | 144500 | 150518 |
| Molecular weight estimates from SAXS MoW analysis | |||
| Mw from Vp (Da) | 199 100 | 89800 | 91800 |
| Nō of Subunits | 4 | 2 | 2 |
| CRYSOL analysis with Tetrameric 3NV8 | |||
| RgE (Å) | 42.4 ± 0.2 | 41.6 ± 0.2 | 29.8 ± 0.2 |
| RgT (Å) | 40.6 | 29.6 ± 0.2 | 39.8 |
| χ | 0.996 | 40.0 | 51.4 |
| CRYSOL analysis with dimer of MtuDAH7PS 3NV8 in asymmetric unit | |||
| RgE (Å) | 41.7 ± 0.2 | 29.6 ± 0.2 | 29.9 ± 0.2 |
| RgT (Å) | 30.7 | 29.1 | 29.3 |
| χ | 13.3 | 0.986 | 2.36 |