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. 2017 Jun 30;12(6):e0180138. doi: 10.1371/journal.pone.0180138

Fig 2. scSOX9 induced collagen type II expression.

Fig 2

Human bone marrow derived mesenchymal stem cells (MSC) was cultured with 10 μg/ml of scGFP (A and D) or scSOX9 (B and E) or a cock tail of growth factor (GF) (C and F) in monolayer (A, B and C) or aggregate (D, E and F) for chondrogenesis. At day 14, aggregates were harvested and snap-frozen. Cryostat sections were stained with a mouse anti-human collage II monoclonal antibody. (Immunoperoxidase staining. Note the poorly formed aggregate by MSC cultured with scGFP only. Representative of 4 experiments). scSOX9: super-charged SRY-type high-mobility group box-9; scGFP: super-charged green fluorescence protein.