Table 2. Selective divalent cations stabilize Cosmc.
| Cosmc ΔTm (°C) | CosmcE152K ΔTm (°C) | CosmcΔ256 ΔTm (°C) | |
|---|---|---|---|
| untreated | 9.6 ± 0.2 | 2.3 ± 1.4 | 1.1 ± 0.5 |
| ZnCl2 | 13.5 ± 0.3 | 7.5 ± 1.2 | -2.2 ± 0.3 |
| FeCl2 | 7.1 ± 0.4 | 2.9 ± 1.6 | -8.8 ± 0.2 |
| MgCl2 | -0.2 ± 0.5 | N.T. | N.T. |
| CaCl2 | -0.9 ± 0.3 | N.T. | N.T. |
| MnCl2 | 0.2 ± 0.2 | N.T. | N.T. |
| CdCl2 | -2.5 ± 0.2 | N.T. | N.T. |
| CoCl2 | -2.6 ± 0.1 | N.T. | N.T. |
| CuCl2 | -7.9 ± 0.3 | N.T. | N.T. |
| NiCl2 | -2.8 ± 0.1 | N.T. | N.T. |
| ATP | -2.5 ± 0.3 | N.T. | N.T. |
| 4MU-GalNAc | -1.4 ± 0.4 | N.T. | N.T. |
| UDP-Gal | 0.4 ± 0.4 | N.T. | N.T. |
ΔTm values for divalent cations are calculated with respect to the EDTA treated samples (ΔTm = Tm − Tm(EDTA treated)). Metals that do not stabilize Cosmc were not tested (N.T.) for CosmcE152K and CosmcΔ256. ΔTm values for ATP, 4MU-GalNAc, UDP-Gal are calculated with respect to untreated samples (ΔTm = Tm − Tm(untreated)).