Fig 8. TEMPOL increases H₂O₂-specific oxidative stress.

A) H₂O₂ levels assessed by Amplex Red Assay in control PASMC in the absence or presence of PEG-catalase (PEG-CAT; 250 U/ml) or TEMPOL (1 mM). SOTS-1 (0.01 mM) is a superoxide donor and used to stimulate increased oxidative stress. Values are means ± SE; n = 5 animals per group; *P ≤ 0.05 vs. vehicle-treated group; # P < 0.05 vs. baseline; analyzed by two-way ANOVA and individual groups compared with the Student-Newman-Keuls test. Representative western blot and summary data showing B) 4-HNE and C) S-sulfenylated proteins in whole lung homogenates from normoxic and hypoxic rats treated with SU5416 and/or TEMPOL. Values are means ± SE; n = 6 animals per group; *P ≤ 0.05 vs. normoxic group; τ p < 0.05 vs. TEMPOL-vehicle group; analyzed by multiple two-way ANOVA and individual groups compared with the Student-Newman-Keuls test.