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. 2017 Jun 30;12(6):e0180558. doi: 10.1371/journal.pone.0180558

Fig 4. Knockdown of c-Fos down-regulated the expression of Wnt2 and its receptor Fzd9 in MG63 cells at 24 and 48 h after transfection with c-Fos siRNA (si-c-Fos) and the negative control (Con).

Fig 4

A. Expression of Wnt2 was examined by RT-PCR. β-actin served as an internal control. B. Expression of Wnt2 was examined by Western blot. GAPDH served as an internal control. C. Data represented means ± SD for four independent experiments of RT-PCR analysis of Wnt2. D. Data represented means ± SD for four independent experiments of Western blot analysis of Wnt2. E. Expression of Fzd9 was examined by RT-PCR. β-actin served as an internal control. F. Expression of Fzd9 was examined by Western blot. GAPDH served as an internal control. G. Data represented means ± SD for four independent experiments of RT-PCR analysis of Fzd9. H. Data represented means ± SD for four independent experiments of Western blot analysis of Fzd9. I. The expression of Wnt2 and its receptor Fzd9 detected by immunofluorescence staining, bar = 50 μm. J. Wnt2 and Fzd9 were enriched in “flow” group, but no bands were found in Co-IP group. No direct connection existed between c-Fos and Wnt2/Fzd9. **p < 0.01, ***p < 0.001 versus negative control (Con).