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. 2017 Jun 30;12(6):e0180578. doi: 10.1371/journal.pone.0180578

Fig 9. Inhibition of soft agar colony formation by the combination of Stat3 and c-Myc siRNA complexed with myr-R9-LyP-1 and E9 peptides.

Fig 9

MDA-MB-231 cells were either left untreated or incubated with control siRNA or a combination of Stat3 and c-Myc siRNAs complexed with either Lf-2000 or myr-R9-LyP-1 and E9 peptides. After 24 hours, the cells were detached with trypsin and replated in soft agar as described in the materials and methods. After 2 weeks, cell colonies larger than 50 cells were quantitated and the results shown. The height of each bar is calculated from the mean of triplicate measurements +/- 1 S.D, and the data analyzed by Analysis of Variance and Tukey's pairwise comparison. Significant differences are indicated (* and ** p < .001). A representative photomicrograph is shown from each condition.