Figure 7. Anti-HA immunolabelling in His6-HA-SUMO1 KI neurons permeabilised with Triton X-100 is mainly nuclear.
Hippocampal neurons from either His6-HA-SUMO1 KI mice or SUMO1 KO mice were fixed in 4% PFA and permeabilised using 0.3% Triton X-100, 10% goat serum, and 0.1% fish skin gelatin for 20 min. Primary antibodies were 1:1000 mouse anti-HA (green), 1:2000 rabbit anti-synapsin 1/2 (red), and 1:500 chicken anti-Map2 (blue). In His6-HA-SUMO1 KI cells, anti-HA labelling was primarily observed in nuclei. Faint anti-HA labelling and occasional puncta were also observed, which did not generally correspond with synapsin-positive puncta (arrowheads). Data representative of 2 independent experiments. Scale bars, 10 µm.