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. 2005 Feb;4(2):337–345. doi: 10.1128/EC.4.2.337-345.2005

FIG. 1.

FIG. 1.

RSM28 alleles affect respiratory growth and mitochondrial reporter activity in cox2-22 and cox2-27 strains. Strains with the indicated nuclear wild-type RSM28, suppressor RSM28-1, or deletion rsm28Δ::URA3 alleles and the indicated mitochondrial alleles in an intact COX2 locus or the cox2::ARG8m reporter fusion were grown as patches on minimal glucose medium and then replica plated to nonfermentable minimal medium for the COX2 alleles (left panels) or minimal glucose medium lacking arginine for the cox2::ARG8m alleles (right panels). Plates were incubated at 16, 30, or 36°C as indicated and were photographed after 2 to 6 days. Strains used (from left to right): COX2; NAB125, NAB126, and NAB86; cox2-22; NAB120, NAB121, and NAB93; cox2-27; NAB122, NAB130, and NAB108; cox2::ARG8m; NAB119, NAB127, and NAB111; cox2-22::ARG8m; NAB123, NAB128, and NAB109; and cox2-27::ARG8m; NAB124, NAB129, and NAB112. As indicated in Table 1, strains lacking a chromosomal URA3 gene were transformed with the URA3 plasmid pDB20 (12).