FIG. 6.

Rsm28p cosediments with the mitochondrial ribosomal small subunit. Mitochondria were isolated from a strain expressing Rsm28p-HA (NAB97). Mitochondrial ribosomes were extracted by treatment with Triton X-100 and sedimented through continuous 15 to 30% sucrose gradients containing 0.5 M NH₄Cl. Alternate fractions of the gradient were precipitated, subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotted, and probed with anti-HA antibody (Rsm28p-HA), anti-Mrp13p (Mrp13p) as a small-subunit standard, anti-Mrp7p (Mrp7p) as a large-subunit standard, and anti-Hsp60p (Hsp60p). M, total mitochondria; P, solubilized sucrose gradient pellet.