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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Cancer Immunol Res. 2017 Mar 6;5(4):330–344. doi: 10.1158/2326-6066.CIR-16-0182

Figure 6.

Figure 6

IDO1 promoter is hypomethylated in basal-like BC subtype. A, Schematic representation of IDO1 gene promoter. B, Methylation status of probe cg10262052 at IDO1 promoter obtained from 450K methylation array data. Student’s t-test was used to compare basal-like vs. lumina A subtypes of breast tumors. C, Inverse correlation between IDO1 promoter methylation and kynurenine levels was observed in the tumors with matched DNA methylation and metabolite profiles. D and E, Heat maps illustrating CpG methylation levels in the IDO1 promoter as determined by pyrosequencing in BC cell lines and primary tumors. F, Intensity plot of CpG methylation levels in BT474-PTEN−LTT and its parental cell lines before and after IFNγ treatment. Immunoblot of IDO1 protein in the same cell line is shown at the bottom. Pyrosequencing data in D, E, and F were acquired from a single experiment. Only low quality data marked by the analysis software were repeated until satisfactory results were obtained.