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. Author manuscript; available in PMC: 2017 Jun 30.
Published in final edited form as: Methods. 2016 Oct 26;113:64–71. doi: 10.1016/j.ymeth.2016.10.013

Fig. 1.

Fig. 1

Molecular basis of differential scanning fluorimetry. (1) Folded protein is incubated in solution with SYPRO Orange dye. (2) Temperature increases, causing protein to unfold and SYPRO Orange dye to bind exposed hydrophobic residues. This results in an increase in fluorescence signal. (3) Protein is denatured and maximum SYPRO Orange dye binding is achieved. (4) Denatured protein begins to aggregate and SYPRO Orange dye binding decreases. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)