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Melting point (Tm) values of ThrRS, HisRS and AlaRS in presence of various substrates.
| Enzyme | Apo Tm (°C) | Amino Acid 5 mM Tm (°C) | ATP 10 mM Tm (°C) | In vitro tRNA 10 uM Tm (°C) |
|---|---|---|---|---|
| ThrRS | 53.9 ± 0.5 | § | 53.8 ± 0.4 | N.D. |
| HisRS | 53.4 ± 1.1 | 57.2 ± 1.1 | 55.8 ± 0.5 | 55.6 ± 0.1 |
| AlaRS | 55.5 ± 0.2 | 55.6 ± 0.1 | 55.0 ± 0.1 | 55.9 ± 0.04 |
Tm ± standard error of the mean.
Multi-phase melting transitions observed. N.D. not determined.
