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Repression of bcl-2 by TSA is an early event and occurs at the transcriptional level. DHL-4 cells were treated with TSA (500 ng/ml), actinomycin D (ActD; 10 μg/ml), or TSA plus ActD for different time periods. Bcl-2 mRNA expression was determined by real-time RT-PCR and normalized to GAPDH expression.
