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. 2005 Mar;25(5):1713–1729. doi: 10.1128/MCB.25.5.1713-1729.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — The ZENON protein is targeted to nuclei. 293T cells were transfected with the complete ZENON coding region (amino acids 1 to 1202) in frame with the FLAG sequence. The ZENON-FLAG fusion protein was detected with an anti-FLAG antibody 48 h after transfection. Nuclei were counterstained with Hoechst reagent. The arrowhead indicates a nucleus containing the ZENON-FLAG fusion protein. The arrow indicates a nucleus in which the ZENON-FLAG fusion protein is not present, probably because the cell was not transfected. Bar, 20 μm.