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. 2005 Mar;25(5):1958–1970. doi: 10.1128/MCB.25.5.1958-1970.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — CENP-H interacts with Hec1. (A) Two-hybrid analysis of interactions between human CENP-H (hCENP-H) and Hec1 (hHec1) (left) and chicken CENP-H (chCENP-H) and Hec1 (chHec1) (right). As a positive control, the interaction between T antigen (AD/T antigen fusion) and p53 (BD/p53 fusion) is shown. AD indicates the GAL4 transcription activation domain, and BD indicates the DNA binding domain of GAL4. Growth was tested on SD minimal plates (−Ade/−His/−Leu/−Trp). (B) Identification of the region of chicken CENP-H required for interaction with chicken full-length Hec1. As bait, constructs of BD fused with CENP-H deletion derivatives were used. As a prey construct, AD fused with chicken Hec1 was used. Growth was tested on SD minimal plates (−Ade/−His/−Leu/−Trp).