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. 2005 Mar;25(5):1958–1970. doi: 10.1128/MCB.25.5.1958-1970.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 5. — Coimmunoprecipitation analysis to investigate the interaction of CENP-H derivatives with Nuf2 complex. Cell lines expressing several CENP-H deletion derivatives were created. Mitotic extracts of these cell lines were immunoprecipitated with anti-Flag antibody. Immunoprecipitates were separated by SDS-PAGE and analyzed by Western blotting with anti-Nuf2 and Hec1 antibodies. One-eighth of each anti-Flag immunoprecipitate was separated by SDS-PAGE and detected with CENP-H as described in the legend of Fig. 4B. IgG, immunoglobulin G.