FIG. 8.

Model of the relation between inner and outer kinetochore structures. The inner kinetochore structure is divided into several subcomplexes that may interact with each other. The CENP-H complex is one such subcomplex. The Nuf2 complex is targeted to the inner kinetochore during G₂ and forms a stable association with the CENP-H complex during mitosis. The Nuf2 complex also interacts with the Mad2 complex, which is a component of the outer kinetochore and is associated transiently with centromeres. In this model, we propose that the Nuf2 complex functions as a connector between inner and outer kinetochores.