TABLE 1.
Complementation and localization analysis of CENP-H derivatives
| CENP-H derivative | Compen- sationa | Localization
|
|
|---|---|---|---|
| Interphase | Metaphase | ||
| Full-length CENP-H-GFP | + | Centromere | Centromere |
| 1-225CENP-H-GFP | + | Centromere | Centromere |
| 1-215CENP-H-GFP | − | Nucleus | Cytoplasm, centrosome |
| 1-185CENP-H-GFP | − | Cytoplasm, centrosome | Cytoplasm, centrosome |
| 1-135CENP-H-GFP | − | Nucleus | Cytoplasm, centrosome |
| 1-85CENP-H-GFP | − | Cytoplasm | Cytoplasm, centrosome |
| 53-235CENP-H-GFP | + | Centromere | Centromere |
| 72-235CENP-H-GFP | + | Centromere | Centromere |
| 83-235CENP-H-GFP | − | Nucleus | Cytoplasm |
| 93-235CENP-H-GFP | − | Nucleus | Cytoplasm |
| 142-235CENP-H-GFP | − | Nucleus | Cytoplasm |
| 72-225CENP-H-GFP | + | Centromere | Centromere |
| 93-185CENP-H-GFP | − | Nucleus | Cytoplasm, centrosome |
a
+ or −, normal growth was or was not observed, respectively, after transfection with the CENP-H derivatives into CENP-H-deficient cells.