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IP-9-induced FAK cleavage is μ-calpain-dependent. EGF (10 nM) and IP-9 (200 ng/ml) stimulation resulted in the appearance of a ≈84-kDa cleavage product of FAK as detected by immunoblotting. The appearance of this fragment was maximal at 30 to 60 min (shown are 30-min challenges). Concomitant treatment with calpain inhibitor 1 (a) or isoform-specific siRNA (b) reduced the level of this cleavage product of FAK. Shown is one of three similar immunoblots for each situation.
