Table 1.
Transgenic phytase crops and their results
| Source | Vector- promoter | Plant system | Result | References |
|---|---|---|---|---|
| Fungi | ||||
| Aspergillus niger | pM0G413- CaMV35s | Tobacco seed | Improved broiler growth | Pen et al. (1993) |
| Aspergillus ficuum | pBI121-CaMV35s | Tobacco | Showed thermo-stability | Wang et al. (2007) |
| Aspergillus niger | pPLEX502 -CaMV 35S & AtPt | Tobacco | Improved P acquisition from soil | George et al. (2009) |
| Aspergillus niger | pAER02 CaMV35s | Tobacco | Proved phytate also is required P acquisition from soil | George et al. (2005) |
| Aspergillus niger | pMOG413 CaMV35s P | Tobacco leaf | Proved phytase stability and can be used as alternative to commercial phytase | Verwoerd et al. (1995) |
| Aspergillus ficuum | pTZ117-CaMV35S | Tobacco leaf | Proved it is equal to commercial phytase | Ullah et al. (1999) |
| Aspergillus ficuum | pPC-KSA-Pky10 | Soybean root | P absorbance efficiency improved | Li et al. (2009) |
| Aspergillus awamori | pBI121-CaMV35s | Soybean | Thermo-stability | Gao et al. (2007) |
| Aspergillus niger | pPhy35s, CaMV35s, seed specific | Soybean seed | Alternative to commercial phytase | Li et al. (1997) |
| Aspergillus niger | pPLEX502 AtPt | Arabidopsis root | Improved P acquisition from soil | Mudge et al. (2003) |
| Aspergillus niger | pUBARN& pUPhyN, Maize ubiquitin-1 | Wheat grain | Phytate degraded into lower forms | Brinch-Pedersen et al. (2000) |
| Aspergillus fumigatus | p1DX5SPConPhyN Wheat HMW 1DX5 GS | Wheat endosperm | Broiler growth was improved like with commercial phytase | Brinch-Pedersen et al. (2006) |
| Aspergillus niger | pSPHP3303T-Phy Maize globulin-1 P | Maize embryo | Phytate content degraded without disturbing seed germination | Chen et al. (2008) |
| Aspergillus niger | pLPL-Rice glutelin-1 | Maize endosperm | 95% P availability increased | Drakakaki et al. (2005) |
| Aspergillus niger | pYU159, Maize ubiquitin | Rice seed | Enhanced inorganic P content | Liu et al. (2006) |
| Aspergillus | pYP46 D35s omega | Canola seed | Proved it can be used as an alternative to the commercial phytase | Peng et al. (2006) |
| Aspergillus ficuum | pTZ117-CaMV35S | Alfalfa leaf | Proved best alternative to the commercial phytase | Ullah et al. (2002) |
| Aspergillus niger | pMOG413-CaMV 35Ps | Sesamame root | Improved P acquisition | Jin et al. (2004) |
| Aspergillus ficuum | pTZ117 CaMV35s promoter | Potato leaves | Proved best alternative to the commercial phytase | Ullah et al. (2003) |
| Aspergillus niger | pBI121-CaMV35s | Brassica napus | Proved recombinant phytase can be best alternative to commercial phytase and improved P acquisition from soil | Wang et al. (2013) |
| Aspergillus niger | p3HB-Kan | Chlamydomonas | It can be best alternative to commercial phytase | Erpel et al. (2016) |
| Bacteria | ||||
| Bacillus subtilus | pCAMBIA1300- CaMV35s | Tobacco | Improved shoot root biomass and IP4/IP5 | Yip et al. (2003) |
| Escherichia coli | pZY101-Soybean lectin | Soybean seed | To mitigate P related problems | Bilyeu et al. (2008) |
| Peniophora lycii | pPLEX502 -CaMV 35S & AtPt | Tobacco | Improved P acquisition from soil | George et al. (2009) |
| Escherichia coli | pCAMBIA2301 Sporamin promoter | Sweet Potato tubers | Improved Plant and animal nutrition | Hong et al. (2008) |
| Escherichia coli | pATPA | Chlamydomonas | Phytate content significantly reduced in broilers excretion | Yoon et al. (2011) |
| Escherichia coli | pBI121-CaMV35s | Brassica napus | Proved recombinant phytase can be best alternative to commercial phytase and Improved P acquisition from soil | Wang et al. (2013) |
| Escherichia coli | Rice | Improved winstar pig nutrition | Cheng-Chih et al. (2008) | |
| Bacillus subtilus | PCx-CAS, 35S Promoter | Tobacco, Arabidopsis | Increased shoot biomass and P acquisition | Lung et al. (2005) |
| Plant and others | ||||
| Arabidopsis thaliana | pBa002a, AtPAP15 | Tobacco | Improved P acquisition from soil | Kuang et al. (2009) |
| Glycine max | pPHY35P-DCaMV 35s | Soybean | To mitigate P related problems | Cheira et al. (2004) |
| Arabidopsis thaliana | pTF101.1-CaMV35S, MtPT1 | Soybean | Improved internal P, plant yield | Wang et al. (2009) |
| Medicago trunculata | pCAMBIA3301-MtPT1 | Arabidopsis root | Fourfold increased fresh and dry weight of the plant | Xiao et al. (2006) |
| Arabidopsis thaliana | pBa002a-AtPAP15 | Arabidopsis | Improved P acquisition from soil | Kuang et al. (2009) |
| Arabidopsis thaliana | pTF101.1-CaMV35S, MtPT1 | Clover | Improved P acquisition | Ma et al. (2009) |
| Oryza sativa | Ole-18 | Rice | Low phytate produced | Ali et al. (2013) |
| Saccharomyces cerevisiae | pOriF, pModF, pOriT-Rice chlorophyll a/b binding (cab) | Rice leaf | Phytase was stable even after 12 weeks of ensilage treatment | Hamada et al. (2005) |