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. 2005 Mar;25(5):1645–1654. doi: 10.1128/MCB.25.5.1645-1654.2005

FIG. 1.

FIG. 1.

Retroviral expression levels of Ik-1 are comparable to expression levels of DNA-binding Ikaros isoforms in wild-type thymocytes. Whole-cell extracts of wild-type thymocytes and purified JE131 cells successfully transduced with the MSCV-(Ik-1)-IRES-H-2Kk (Ik-1) or the MSCV-(Ik-7)-IRES-H-2Kk (Ik-7) retrovirus were prepared. A total of 10 μg of protein was subjected to SDS-PAGE, and Western blotting was performed with an anti-Ikaros monoclonal antibody. The two Ikaros bands visible in the wild-type thymocytes correspond to the predominantly expressed Ikaros isoforms Ik-1 (top band) and Ik-2 and Ik-3, which comigrate (bottom band). The Ik-1 band in the retrovirally transduced cells is of slightly larger molecular weight due to the Flag epitope tag. Densitometry readings are shown under each band. This figure was generated by using Adobe Photoshop software. Thy, thymocytes; Ab, antibody.