Figure 7.

Representative B-cell gating plots and subsets. CD19⁺CD20⁺ and CD19⁺CD20⁻ B-cell populations (a) were identified within CD45⁺ lymphocytes. CD19⁺CD20⁺ were subsequently divided into switched memory (SM: IgD⁻CD27⁺), non-switched memory (NSM: IgD⁺CD27⁺) and DNB (IgD⁻CD27⁻) (b). Cells of an IgD⁺CD27⁻ phenotype were further divided into naive (CD19⁺CD20⁺IgD⁺CD27⁻CD24^loCD38^lo), CD24^hiCD38⁻ (CD19⁺CD20⁺IgD⁺CD27⁻CD24^hiCD38⁻) and transitional (CD19⁺CD20⁺IgD⁺CD27⁻CD24^hiCD38^hi) subsets (c). Antibody-secreting cells (ASC: CD19⁺CD20⁻CD27^hiCD38^hiIgD⁻CD24⁻) were identified within the CD19⁺CD20⁻ B-cell population by first gating CD27^hiCD38^hi cells (d), then confirming their IgD⁻CD24⁻ phenotype (e).