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. 2017 Jun 30;7:4405. doi: 10.1038/s41598-017-04492-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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sWAT characterization: Hyperproliferation, senescence and DNA damage. (A) Proliferation was determined by immunofluorescent staining of Ki67 (red) in cell nuclei (blue), subsequently analyzed as the percentage of positively stained cells in mutant and control sWAT. (B) Senescence was determined via quantitative RT-PCR of specific target genes. (C,D) γH2AX immunofluorescent staining showing DNA DSBs (red) in cell nuclei (blue) of wild-type and RPE sWAT, first analyzed for the frequency of positive cells (C) and then for the frequency of γH2AX foci per cell (D). Scale bars: A = 50 μm, C = 10 μm. Data are represented as mean ± SEM.