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. 2005 Mar;25(5):1696–1712. doi: 10.1128/MCB.25.5.1696-1712.2005

FIG. 6.

FIG. 6.

Phosphorylated Akt1 is expressed in the epithelial compartment of developing hair follicles. (A) The spatiotemporal expression pattern of phosphorylated Akt1 at both T308 (Akt1-T308-P) and S473 (Akt1-S473-P) in the developing postnatal mouse skin (P1 to P10) was assessed by immunofluorescence. Phosphorylated Akt1 colocalizes with PPARβ in hair placodes (PL), hair germs (HG), and hair pegs (HP) of developing HFs at early stages (P1 to P4), and then became restricted to ORS keratinocytes of mature HFs (ORS; P7to P10), with no detectable expression in hairmatrix (HM; P10) and dermal papilla cells (DP; P10). (B) Skin sections of PPARβ+/+ and PPARβ−/− mice were compared at P4 for the presence of phosphorylated Akt1 and phosphorylated GSK-3β at S9 (GSK-3β-S9). Reduced immunostaining of phosphorylation sites of both Akt1 and GSK-3β was observed in follicular keratinocytes of PPARβ−/− skin (asterisks). Arrowheads indicate the expression of GSK-3β-S9 in the hair shaft precursors (precortex region) of differentiating HFs. Cell nuclei were counterstained with DAPI. Magnification bars, 50 μm.