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. 2017 Jun 30;7:4416. doi: 10.1038/s41598-017-03733-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced activity of purified PIV from QS mutant. PIV was purified from PAO-T7 (P-PIV) and PAO-T7-MW1 (M-PIV) and the purity was confirmed in SDS-PAGE (A). PIV bands at 26 kDa are indicated. (B) the activity of P-PIV and M-PIV was measured at 37 °C using chromogenic substance. Same amount of M-PIV and P-PIV (100 ng) were injected to T. moliter larvae and death with melanization was observed (C). Same volume of insect saline (IS) and protein storage buffer (0 ng) were injected as controls. The survival along with days (D) and at day 4 (E) was quantified. *p < 0.01.