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. 2017 Jun 30;7:4416. doi: 10.1038/s41598-017-03733-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Propeptide degradation and activity restoration of M-PIV by LasB. (A) The purified 100 ng of LasB was mixed with 800 ng of M-PIV in 25 μl reaction volume and incubated at room temperature (RT) for 30 min. For control, the purified LasB and M-PIV were separately incubated in the same condition. All fractions were applied to 12% SDS-PAGE and visualized by Coomassie staining. Lane 1–2, M-PIV alone with no or 30 min incubation; lane 3–4, LasB alone with no or 30 min incubation; lane 5–7, M-PIV + LasB with no, 5, or 30 min incubation. This gel picture was cropped and the full-length gel picture is presented in supplementary data (Fig. S4B). (B) The PIV activities of P-PIV, M-PIV, LasB, and M-PIV + LasB were measured by chromogenic substrate. The amount of the purified proteins used in each reaction is indicated. The activity was measured promptly after adding the proteins without incubation.