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. 2017 Jun 30;7:4469. doi: 10.1038/s41598-017-04736-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RAD51-AS1 knockdown regulates gene expression in SKOV3.ip cells. (A), Hierarchical clustering of the genes differentially expressed in SKOV3.ip cells 24 hours after RAD51-AS1 silencing. Each column represents one sample and each row represents one gene. The color scale at the top of the figure illustrates the relative expression of a gene across all of the samples, with red indicating high expression and green indicating low expression. (B), Volcano plot shows that some genes were differentially expressed after RAD51-AS1 knockdown. (C), Analysis of pathway relation network. The interactions in the KEGG Database were used to construct the interaction network between pathways, and the top ten pathways after silencing RAD51-AS1 are shown. (D,E and F), The significant analysis of Gene Ontology (GO) on three aspects (D, biological process analysis; E, molecular function analysis; F, cellular component analysis) were performed with parameter settings of P = 0.05, FDR = 0.05, and the top ten terms are listed.