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. 2017 Jun 30;7:4420. doi: 10.1038/s41598-017-04655-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in total boron concentration (A) and concentration of different forms (B) of cotton leaves after formation of brown rings on the petiole under boron deficiency compared with normal leaves (control). Total (B) was extracted from dry sieved functional leaves with 1 M HCl. Different forms of (B) were extracted from fresh functional leaves following a three-step procedure. The data of six replicates are expressed as the mean + SE.